PROTEIN PRODUCTION BY RECOMBINANT DNA TECHNOLOGY

Course Description

In Protein Production by Recombinant DNA Technology course, Recombinant DNA technology production techniques like PCR, Molecular Cloning, Restriction enzymes, plasmids will be discussed in addition to the work flow. General information about the protein extraction and purification techniques, expression systems, fermentation techniques, fermentors and bioreactors, up and downstream processes, separation and purification techniques for expressed proteins and production related problems will be covered. Utilization of different organisms and microorganisms for protein production as well as protein engineering techniques will be discussed. CRIPR-Cas system and novel techniques and technologies in genetic and protein engineering as well as the basic biotechnological methods are among the covered topics.

PROTEIN PRODUCTION BY RECOMBINANT DNA TECHNOLOGY

Evaluation Tools (Active Term)

No evaluation items have been defined.

Course outcomes

1. 01 Students are expected to: obtain an understanding of critically evaluating primary research articles
2. 02 Learn the details of structure and function of nucleic acids
3. 03 Obtain an understanding of molecular cloning strategies
4. 04 Learn about protein expression, purification, and analysis techniques
5. 05 Learn about drugs obtained via recombinant DNA technology

Course Syllabus

Reference Books & Course Materials

1. 01 FUNDAMENTALS OF BIOCHEMISTRY by VOET, VOET, PRATT, Upgrade Edition, ISBN:0-471-41759-9
2. 02 An Introduction to Genetic Introduction by Desmond Nicholl, 3rd ed. ISBN-13: 978-0521615211

Learning Outcomes

1. L01 Students are expected to: obtain an understanding of critically evaluating primary research articles SOLO 5
2. L02 Identify and demonstrate the details of structure and function of nucleic acids SOLO 3
3. L03 Implement molecular cloning strategies SOLO 4
4. L04 Explain, design and apply about protein expression, purification, and analysis techniques/procedures SOLO 4
5. L05 Explain drugs obtained via recombinant DNA technology SOLO 3

Program Outcomes

1. P01 Gain the ability to apply the Pharmacy profession.
2. P02 Interpret the accuracy and reliability of the current theoretical and practical knowledge in the field of pharmacy, as well as being able to follow and evaluate innovations.
3. P03 Practically apply the knowledge they gain on pharmaceutical sciences. The acquired knowledge and skills allow the graduates to act as the closest health consultant to the public with the ability to inform the public.
4. P04 Evaluate and apply current technology related to basic subjects and principles covering pharmacy and health sciences and take part in in-service training seminars.
5. P05 Know the risks and evaluation of the use of drugs and other chemicals and safe laboratory practices (GMP).
6. P06 Acquire the ability to interpret the findings obtained by qualitative and quantitative measurements and to reach valid scientific results from the data.
7. P07 Prepare pharmaceuticals and provide guidance on the use of pharmaceutical products.
8. P08 Design research and develop projects related to pharmaceutical products; manage and share the results.
9. P09 Be competent to accurately and intelligibly communicate and transfer knowledge about prescription content and pharmaceutical products to the patient / user. The graduates are trained on taking necessary precautions for safe drug usage and encouraging rational drug use, as well as providing consultancy and supervision.
10. P10 Work in an interdisciplinary environment. Graduates can cooperate with other professional groups, participate in vocational training and activities, have proper knowledge and communication skills.
11. P11 Take part in social responsibility projects related to health. Graduates have awareness on sustainable environment and participate in activities and practices related to their profession.
12. P12 Open a community pharmacy, also work in hospital pharmacies, can work in different fields related with pharmaceuticals, cosmetics, medical and herbal products, as well as production and quality control in the pharmaceutical industry. Takes part in the professional development of herself/himself, her/his colleagues and pharmacy students.
13. P13 Implements the legal and professional law and ethics as a health professional, in issues concerning public health.
14. P14 Knows and applies the processes related to drug design from natural or synthetic sources, as well as development, preparation, analysis and metabolism of drugs in the human body.

Po-Lo Matrix