PLANT TISSUE CULTURE TECHNIQUES

Course Description

Historical background of tissue culture studies. Important expressions of the tissue cultures. Importance of chemicals used in tissue culture media and their properties. Media preparations and their constitutes. Calculation of possible modifications in media. Tissue culture stages. Selection of explants sources or mother plants and their properties. Elimination of virus and other pathogens in plants. Preventing or elimination of oxidation of possible oxidations. Use of active charcoal and ascorbic asid. Preparations of explants, sterilization, initiation, multiplications and rooting processes. Adaptation of plantlets to the greenhouse environments and open fields. Physical control of growth rooms. Designing tissue culture laboratories, necessary equipments and their properties. Education of personel in the lab personel.

PLANT TISSUE CULTURE TECHNIQUES

Evaluation Tools (Active Term)

Course outcomes

1. 01 Describe (3) conventional plant propagation methods
2. 02 Explain (3) the advantages and disadvantages of tissue culture methods
3. 03 Perform (4) the physical laboratory control requirements inherent to palnt tissue culture
4. 04 Implement (4) the different stages of tissue culture
5. 05 Discuss (5) the advantages and disadvantages of tissue culture methods

Course Syllabus

Reference Books & Course Materials

1. 01 Roberta, smith, 2012, Plant tissue Culture: Techniques and experiments, Academic Press 3rd Edition, ISBN-13: 978-0124159204
2. 02 Bhojwani, Sant Saran, Dantu, Prem Kumar, 2013, Plant Tissue Culture: An Introductory Text, Springer, ISBN 978-81-322-1026-9
3. 03 Trigiano, R. N., & Gray, D. J. (Eds.). (2011). Plant tissue culture, development, and biotechnology. CRC Press. 1st ed., Boca Raton (FL); xviii + 583 pp. + CD-ROM; ISBN 978-1-4200-8326-2.
4. 04 George, E. F., Hall, M. A., & De Klerk, G.-J. (Eds.). (2008). Plant propagation by tissue culture: Vol. 1. The background (3rd ed.). Springer. Dordrecht; ISBN 978-1-4020-5004-6 (hardcover); DOI: 10.1007/978-1-4020-5005-3; xii + 502 pp.

Learning Outcomes

1. L01 Describe (3) conventional plant propagation methods SOLO 3
2. L02 Explain (3) the advantages and disadvantages of tissue culture methods SOLO 3
3. L03 Perform (4) the physical laboratory control requirements inherent to plant tissue culture SOLO 4
4. L04 Implement (4) the different stages of tissue culture SOLO 4
5. L05 Implement (4) virus-free plant production techniques SOLO 4

Program Outcomes

1. PCST PÇ1: Develop and deepen knowledge acquired in plant science and technologies based on undergraduate level competencies.
2. PCST PÇ2: Understand the interaction between plant science and technologies field and related disciplines.
3. PCST PÇ3: Combine and interpret the theoretical and practical knowledge related to horticultural plants with the knowledge, data and findings obtained from different disciplines, to be able to create new knowledge and theories by synthesizing them by using the theoretical and practical knowledge related to horticultural plants in the field of expertise and supporting the current developments with quantitative and qualitative.
4. PCST PÇ4: Solve problems by using research methods and solving cause and effect relationships.
5. PCST PÇ5: Conduct a study requiring expertise on plant science and technologies independently.
6. PCST PÇ6: Develop an analytical approach for solving unpredictable complex problems encountered in applications of plant science and technologies, to design the research process, to produce solutions by taking responsibility and to evaluate and defend the results obtained.
7. PCST PÇ7: Lead the plant science and technologies in an environment that requires solving problems.
8. PCST PÇ8: Access to resources related to plat science and technologies, benefiting from these resources and self-renewal.
9. PCST PÇ9: Transfer his / her studies, developments in his / her field of expertise and research results using oral, written and visual tools.
10. PCST PÇ10: Use advanced computer software and information and communication technologies as necessary in relation to plant science and technologies.
11. PCST PÇ11: Collect data with scientific methods for solving problems related to plant science and technologies, to be able to control and interpret the collected data by taking into consideration the social, scientific and ethical values.
12. PCST PÇ12: Utilize and explain integrated knowledge in plant science and technologies in interdisciplinary studies, communicate it effectively, and critically evaluate the conclusions drawn.

Po-Lo Matrix