PLANT TISSUE CULTURE TECHNIQUE
- Course
- PST313 - PLANT TISSUE CULTURE TECHNIQUE
- Department
- Plant Production and Technology - English - Undergraduate
- Course Type
- Course
- Status
- Required
- Language
- English
- Credit
- 3
- ECTS
- 3
- T+P+L
- 3 + 0 + 0
- Course Coordinator(s)
- -
- Prerequisite
- -
- Keywords
Course Description
"Objective of this course is to give theoretical knowledge and application techniques in tissue culture area. Course covers; Organization of Plant Tissue Culture Laboratory, Aseptic techniques, nutrition and media, Callus culture, Cell cultures, Protoplast cultures, Somatic embryogenesis, Organogenesis, Root cultures, Meristem culture and elimination of viruses, Clonal propagation, shoot tip culture, adventitious root. "
PLANT TISSUE CULTURE TECHNIQUE
Evaluation Tools (Active Term)
No evaluation items have been defined.
Course outcomes
No course outcomes have been defined yet.
Course Syllabus
| Week | Topic |
|---|---|
| Week 1 | Introduction to course, histroricak background and necessary definations |
| Week 2 | Advantages and disadvantages of tissue culture techniques in plant propagation |
| Week 3 | Stages of tissue culture methods |
| Week 4 | Sterilizastion practices and methods |
| Week 5 | Tissue culture labıratories and necessary equipmenyts |
| Week 6 | Tissue culture media and Murashige and Skkog Medium (MS medium), stock solutions |
| Week 7 | Midterm |
| Week 8 | Physical conditions of growing cells (rooms): light, temperature, humidity, ventilation |
| Week 9 | How to obtain virus -free starting plants |
| Week 10 | Controlling possible disease agents and insect damages in both glasses and cells |
| Week 11 | Discarding infected materials and sterilizations |
| Week 12 | Adapting plantlets to the greenhouse and outdoor conditions- acclimatization |
| Week 13 | Grading, packing and transfering the tissue culture propagated plants |
| Week 14 | Drawing a small tissue culture laboratory and overview the corse |
| Week 15 | - |
Reference Books & Course Materials
- 01 H. S. Roberts, 2012, Plant Tissue Culture: Techniques and Experiments, 3 rd. Ed., Academic Press
- 02 Archana, 2015. Tissue Culture for Plant Propagation. AbeBooks, Amazon. ISBN-10: 1634155120
Learning Outcomes
No learning outcomes have been defined.
Program Outcomes
- P01 Becoming aware of sufficient theoretical knowledge in basic engineering and agricultural science
- P02 Having sufficient theoretical knowledge in basic agricultural production especially in pomology, viticulture, olericulture, floriculture and agronomy, and their breeding
- P03 Being well equiped with practical experiences in agriculture
- P04 Finding the source of environmental, biotic, abiotic, technical and economical problems in agricultural crops which reduce yield and quality, and create solutions to related problems
- P05 Defining, classifying and naming cultivated crop plants and knowing how to manage them
- P06 Breeding of horticultural and field crops, developing new cultivars and producing propagation materials of new developed varieties
- P07 Applying up-to-date methods and techniques for preserving genetic resources and environment for sustainable use in agricultural commodities
- P08 Evaluating, asseying and analyzing the changes in cultivation of horticultural and agronomic crops in respect to irrigation, fertilization, defining pests and diseases and after postharvest handling
- P09 Establishing and managing orchards, vineyards and field crop productions and greenhouse operations
- P10 Obtaining data in horticulture and field crops along with researching, considering, recording, consulting and expert abilities, project writing and applying
- P11 Identifying different roles in enchancing individual abilities and skills beside taking responsibilities, participations in team works under any given conditions
- P12 Easily accessing to appropriate information and thinking analytically in their interested area and subjects. They can do accurate timing and planning in agricultural productions in year round. They think analytically, prepare questionaries, have an exploratory and creative vision and use the obtained knowledge for the benefit of farmers and societies
- P13 Having ability to transfer information and also able to easily communicate with the people in the field at different levels. They have self - confidence and self- improvement skills as well
Po-Lo Matrix
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