Skip to main content
TR

CELL CULTURE TECHNIQUES

Course
PHAR424 - CELL CULTURE TECHNIQUES
Department
Pharmacy - English - Undergraduate
Course Type
Course
Status
Required
Language
English
Credit
2
ECTS
0
T+P+L
2 + 0 + 0
Course Coordinator(s)
-
Prerequisite
-
Keywords

Course Description

In the Cell Culture Techniques course, introduction of cell culture, cell culture laboratory layout and equipment used in cell culture, types and origins of cells used in culture, cell bank systems, cell bank system applications, cell freezing and storage methods, cell bank system applications, cell viability and toxicity tests, tissue engineering are planned as the main topics. Application of these techniques to pharmaceutical sciences is also part of the course’s aims. Use of cell culture techniques in current and novel technological advances including vaccine generation purposes, gene therapy methods, drug development and testing, bacterial and viral infectivity studies, stem cell technology, different cell culture types and their use in various experimental studies are covered.

CELL CULTURE TECHNIQUES

Evaluation Tools (Active Term)

No evaluation items have been defined.

Course outcomes

  1. 01 Identify the basics consepts of cell culture techniques
  2. 02 Compare and describe different contaminations and sterilisation methods
  3. 03 Calculate the number of cells in hemocytometer
  4. 04 Describe cell culture media and supplements

Course Syllabus

Week Topic
Week 1 Introduction to Cell Culture
Week 2 Laboratory Design, Layout, and Equipment
Week 3 Equipment and Materials
Week 4 Aseptic Technique
Week 5 Sterilisation Techniques
Week 6 Defined Media and Supplements
Week 7 Revision
Week 8 Midterm
Week 9 Midterm
Week 10 Serum-Free Media, Culture Vessels and Substrates
Week 11 Primary Culture
Week 12 Subculture and Cell Lines
Week 13 Basic Cell Culture Methods
Week 14 Revision for final exam
Week 15 Final Exam

Reference Books & Course Materials

  1. 01 Culture of animal cells, R. Ian Freshney, 6th edition, A John Wiley & Sons, Inc, Publication, 2010
  2. 02 Animal Cell Culture and Technology, Michael Butler, Garland Science/BIOS Scientific Publishers, 2004

Learning Outcomes

No learning outcomes have been defined.

Program Outcomes

  1. P01 Gain the ability to apply the Pharmacy profession.
  2. P02 Interpret the accuracy and reliability of the current theoretical and practical knowledge in the field of pharmacy, as well as being able to follow and evaluate innovations.
  3. P03 Practically apply the knowledge they gain on pharmaceutical sciences. The acquired knowledge and skills allow the graduates to act as the closest health consultant to the public with the ability to inform the public.
  4. P04 Evaluate and apply current technology related to basic subjects and principles covering pharmacy and health sciences and take part in in-service training seminars.
  5. P05 Know the risks and evaluation of the use of drugs and other chemicals and safe laboratory practices (GMP).
  6. P06 Acquire the ability to interpret the findings obtained by qualitative and quantitative measurements and to reach valid scientific results from the data.
  7. P07 Prepare pharmaceuticals and provide guidance on the use of pharmaceutical products.
  8. P08 Design research and develop projects related to pharmaceutical products; manage and share the results.
  9. P09 Be competent to accurately and intelligibly communicate and transfer knowledge about prescription content and pharmaceutical products to the patient / user. The graduates are trained on taking necessary precautions for safe drug usage and encouraging rational drug use, as well as providing consultancy and supervision.
  10. P10 Work in an interdisciplinary environment. Graduates can cooperate with other professional groups, participate in vocational training and activities, have proper knowledge and communication skills.
  11. P11 Take part in social responsibility projects related to health. Graduates have awareness on sustainable environment and participate in activities and practices related to their profession.
  12. P12 Open a community pharmacy, also work in hospital pharmacies, can work in different fields related with pharmaceuticals, cosmetics, medical and herbal products, as well as production and quality control in the pharmaceutical industry. Takes part in the professional development of herself/himself, her/his colleagues and pharmacy students.
  13. P13 Implements the legal and professional law and ethics as a health professional, in issues concerning public health.
  14. P14 Knows and applies the processes related to drug design from natural or synthetic sources, as well as development, preparation, analysis and metabolism of drugs in the human body.

Po-Lo Matrix

The PO-LO matrix has not been populated yet.