MOLECULAR BIOLOGY LAB I

Course Description

The course provides both theoretical and practical in depth explanations of several molecular biology concepts and laboratory techniques. The main topics of this course include analyses of structure and function of various enzymes used in molecular biology, detailed presentations on DNA/ RNA purification, DNA/RNA analyses and calculations, different types of PCRs and their uses, the applications and methods of DNA cloning, design and functions of plasmid and mammalian vectors and how they are used in research, yeast two hybrid systems, the design and uses of DNA libraries, history of DNA sequencing, uses and technological advancements. Throughout the course, data from primary literature are analyzed and practice problems are solved.

MOLECULAR BIOLOGY LAB I

Evaluation Tools (Active Term)

No evaluation items have been defined.

Course outcomes

No course outcomes have been defined yet.

Course Syllabus

Reference Books & Course Materials

1. 01 Molecular Biology Techniques: A Classroom Laboratory Manual,  Carson et al. 978-0123855459
2. 02 Genetics: From Genes to Genomes, Hartwell et al. 978–1259700903

Learning Outcomes

1. L01 Able to recall and define lab safety rules, regulations, and materials. SOLO 2
2. L02 Performing accurate micro pipetting and explaining the reason of pipette calibration. SOLO 4
3. L03 Applying theoretical knowledge of RNA/DNA extraction method and accurate performing of the experiment. SOLO 4
4. L04 Application of PCR technique, performing agarose gel electrophoresis techniques according to specific methodology and visualisation of DNA/RNA. SOLO 4
5. L05 Ability to define molecular background of COVID-19 and discuss the COVID-19 diagnosis methodology SOLO 3.5
6. L06 Able to explain the role of genes in phenotype expression and also analyze the PTC gene using Rt-PCR SOLO 4
7. L07 Able to explain restriction enzymes and cloning techniques. Then, able to transfer a plasmid into bacterial culture and develop a recombinant DNA SOLO 4.5
8. L08 Able to discuss DNA fingerprinting in forensics and southern blot methodolgy and perform southern blot analysis SOLO 4.5

Program Outcomes

1. P01 PO1: To identify and use theoretical and applied knowledge as well as up-to-date/cutting edge laboratory techniques and analyses methods in the field of molecular biology and genetics.
2. P02 PO2: To identify problems related to molecular biology and genetics.
3. P03 PO3: To design and conduct experiments through hypothesis driven scientific research; and to analyze and interpret data to find solutions to molecular biology and genetics related problems.
4. P04 PO4: To work independently and as part of research group in a multidisciplinary team setting
5. P05 PO5: To apply the principles of scientific research ethics and academic integrity
6. P06 PO6: To interpret, explain and present primary scientific literature and novel findings in oral, written and visual presentation formats
7. P07 PO7: To evaluate experimental data and theoretical findings by using critical and analytical thinking techniques.
8. P08 PO8: To use the currently acceptable process of preparing research proposals and manuscripts for publishing in scientific journals.
9. P09 PO9: To apply the principles of molecular biology and genetics in generating/finding solutions to social problems / issues.
10. P10 PO10: To identify and implement environmental and laboratory safety procedures.
11. P11 PO11: To identify and apply the necessary professional skill set in projects and jobs involving independent and competitive careers in academia, industrial, medical or clinical research, science education, scientific writing and communication, health care, health care policy and civil service.

Po-Lo Matrix