GENETIC ENGINEERING

Course Description

The main focus of the course is on the fundamental principles and techniques used in gene manipulation. The course provides an introduction to the fundamentals of molecular biology, the methods used to manipulate genes, and applications of the technology. During this course basic features of genetic engineering, emergence and historical development of gene manipulation technology, genomic organisation, gene expression and regulation, techniques for isolation, handling, and processing of nucleic acids, principles of nucleic acid hybridisation, gel electrophoresis techniques, and DNA sequencing, enzymes used in gene manipulation, features of plasmid and bacteriophage vectors and DNA sequence cloning strategies will be covered.

GENETIC ENGINEERING

Evaluation Tools (Active Term)

Course outcomes

1. 01 Identify The way that living systems are organised, the flow of genetic information, the structure of DNA and RNA, gene organisation, gene expression, genes and genomes.
2. 02 Describe nucleic acid hybridisation, gel electrophoresis and DNA sequencing
3. 03 Explain what is genetic engineering.
4. 04 Explain getting DNA into cells.
5. 05 List the laboratory requirements,techniques used in iIsolation of DNA and RNA, handling and quantification of nucleic acids and labelling nucleic acids
6. 06 Explain cloning from genomic DNA.
7. 07 Summarize Restriction enzymes, DNA modifying enzymes and DNA ligase.
8. 08 Summarize advanced cloning strategies.
9. 09 Elaborate on which cloning approach is best and cloning from mRNA.
10. 10 Elaborate on host cell types, plasmid vectors for use in E. Coli, bacteriophage vectors for use in E. coli.

Course Syllabus

Reference Books & Course Materials

1. 01 Desmond S. T. Nicholl. An Introduction to Genetic Engineering (3rd edition). Cambridge University Press, 2008.

Learning Outcomes

1. L01 Identify The way that living systems are organised, the flow of genetic information, the structure of DNA and RNA, gene organisation, gene expression, genes and genomes. SOLO 2
2. L02 Describe nucleic acid hybridisation, gel electrophoresis and DNA sequencing SOLO 3
3. L03 Explain what is genetic engineering. SOLO 3
4. L04 Explain getting DNA into cells. SOLO 3
5. L05 List the laboratory requirements,techniques used in iIsolation of DNA and RNA, handling and quantification of nucleic acids and labelling nucleic acids SOLO 3
6. L06 Explain cloning from genomic DNA. SOLO 3
7. L07 Summarize Restriction enzymes, DNA modifying enzymes and DNA ligase. SOLO 4
8. L08 Summarize advanced cloning strategies. SOLO 4
9. L09 Elaborate on which cloning approach is best and cloning from mRNA. SOLO 5
10. L10 Elaborate on host cell types, plasmid vectors for use in E. Coli, bacteriophage vectors for use in E. coli. SOLO 5

Program Outcomes

1. P01 PO1: To identify and use theoretical and applied knowledge as well as up-to-date/cutting edge laboratory techniques and analyses methods in the field of molecular biology and genetics.
2. P02 PO2: To identify problems related to molecular biology and genetics.
3. P03 PO3: To design and conduct experiments through hypothesis driven scientific research; and to analyze and interpret data to find solutions to molecular biology and genetics related problems.
4. P04 PO4: To work independently and as part of research group in a multidisciplinary team setting
5. P05 PO5: To apply the principles of scientific research ethics and academic integrity
6. P06 PO6: To interpret, explain and present primary scientific literature and novel findings in oral, written and visual presentation formats
7. P07 PO7: To evaluate experimental data and theoretical findings by using critical and analytical thinking techniques.
8. P08 PO8: To use the currently acceptable process of preparing research proposals and manuscripts for publishing in scientific journals.
9. P09 PO9: To apply the principles of molecular biology and genetics in generating/finding solutions to social problems / issues.
10. P10 PO10: To identify and implement environmental and laboratory safety procedures.
11. P11 PO11: To identify and apply the necessary professional skill set in projects and jobs involving independent and competitive careers in academia, industrial, medical or clinical research, science education, scientific writing and communication, health care, health care policy and civil service.

Po-Lo Matrix