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Cyprus International University
TR

GENETIC ENGINEERING

Course
BIOE403 - GENETIC ENGINEERING
Department
Bioengineering - English - Undergraduate
Course Type
Course
Status
Required
Language
English
Credit
4
ECTS
6
T+P+L
4 + 0 + 0
Course Coordinator(s)
Asst. Prof. Dr. Nihal BAYIR
Prerequisite
Keywords

Course Description

The main focus of the course is on the fundamental principles and techniques used in gene manipulation. The course provides an introduction to the fundamentals of molecular biology, the methods used to manipulate genes, and applications of the technology. During this course basic features of genetic engineering, emergence and historical development of gene manipulation technology, genomic organisation, gene expression and regulation, techniques for isolation, handling, and processing of nucleic acids, principles of nucleic acid hybridisation, gel electrophoresis techniques, and DNA sequencing, enzymes used in gene manipulation, features of plasmid and bacteriophage vectors and DNA sequence cloning strategies will be covered.

GENETIC ENGINEERING

Evaluation Tools (Active Term)

Item Type Weight (%)
Presentation Presentation 15
Midterm Exam Midterm 35
Final Exam Final 50
Total 100

Course outcomes

  1. 01 Identify The way that living systems are organised, the flow of genetic information, the structure of DNA and RNA, gene organisation, gene expression, genes and genomes.
  2. 02 Describe nucleic acid hybridisation, gel electrophoresis and DNA sequencing
  3. 03 Explain what is genetic engineering.
  4. 04 Explain getting DNA into cells.
  5. 05 List the laboratory requirements,techniques used in iIsolation of DNA and RNA, handling and quantification of nucleic acids and labelling nucleic acids
  6. 06 Explain cloning from genomic DNA.
  7. 07 Summarize Restriction enzymes, DNA modifying enzymes and DNA ligase.
  8. 08 Summarize advanced cloning strategies.
  9. 09 Elaborate on which cloning approach is best and cloning from mRNA.
  10. 10 Elaborate on host cell types, plasmid vectors for use in E. Coli, bacteriophage vectors for use in E. coli.

Course Syllabus

Week Topic
Week 1 Introduction to course organisation
Week 2 What is genetic engineering? Laying the foundations. First steps.
Week 3 The way that living systems are organised. The flow of genetic information. The structure of DNA and RNA. Gene organisation. Gene expression. Genes and genomes.
Week 4 Laboratory requirements, Isolation of DNA and RNA. Handling and quantification of nucleic acids. Labelling nucleic acids
Week 5 Nucleic acid hybridisation. Gel electrophoresis. DNA sequencing
Week 6 Restriction enzymes -- cutting DNA
Week 7 Review
Week 8 MID-TERM EXAMINATIONS
Week 9 DNA modifying enzymes. DNA ligase -- joining DNA molecules.
Week 10 Host cell types. Plasmid vectors for use in E. coli. Bacteriophage vectors for use in E. coli.
Week 11 Other vectors. Getting DNA into cells.
Week 12 Which cloning approach is best? Cloning from mRNA.
Week 13 Cloning from genomic DNA. Advanced cloning strategies.
Week 14 Review
Week 15 Final Exams.

Reference Books & Course Materials

  1. 01 Desmond S. T. Nicholl. An Introduction to Genetic Engineering (3rd edition). Cambridge University Press, 2008.

Learning Outcomes

  1. L01 Identify The way that living systems are organised, the flow of genetic information, the structure of DNA and RNA, gene organisation, gene expression, genes and genomes. SOLO 2
  2. L02 Describe nucleic acid hybridisation, gel electrophoresis and DNA sequencing SOLO 3
  3. L03 Explain what is genetic engineering. SOLO 3
  4. L04 Explain getting DNA into cells. SOLO 3
  5. L05 List the laboratory requirements,techniques used in iIsolation of DNA and RNA, handling and quantification of nucleic acids and labelling nucleic acids SOLO 3
  6. L06 Explain cloning from genomic DNA. SOLO 3
  7. L07 Summarize Restriction enzymes, DNA modifying enzymes and DNA ligase. SOLO 4
  8. L08 Summarize advanced cloning strategies. SOLO 4
  9. L09 Elaborate on which cloning approach is best and cloning from mRNA. SOLO 5
  10. L10 Elaborate on host cell types, plasmid vectors for use in E. Coli, bacteriophage vectors for use in E. coli. SOLO 5

Program Outcomes

No program outcomes have been defined.

Po-Lo Matrix

The PO-LO matrix has not been populated yet.